Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR
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چکیده
منابع مشابه
Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR.
Lawsonia intracellularis is not culturable with a standard bacteriologic culture. One step PCR assay as a clinical diagnostic method was developed for the rapid detection of porcine proliferative enteritis (PPE) caused by L. intracellularis. Primers were designed based on the p78 DNA clone of L. intracellularis. The one step PCR resulted in the formation of a specific 210-bp DNA product derived...
متن کاملRapid Detection of Brachyspira Hyodysenteriae and Lawsonia Intracellularis in Swine Faecal and Mucosal Specimens by Multiplex Pcr
Multiplex polymerase chain reaction (M-PCR) was developed to determine its usefulness as a rapid and specific method for routine diagnosis of Brachyspira hyodysenteriae and Lawsonia intracellularis infections in pigs. The assay was based on simultaneous amplification of the tlyA-encoded haemolysin gene of B. hyodysenteriae and 16S rRNA sequences of L. intracellularis. The results of this study ...
متن کاملThe discovery of Lawsonia intracellularis
Lawson, G. H. K.; McOrist, S.; Sabri, J. and Mackie, R. A. (1993): Intracellular bacteria of porcine proliferative enteropathy: Cultivation and maintenance in vitro. J Clin Microbiol 31: 1136 – 1142. McOrist, S.; Jasni, S.; Mackie, R. A.; MacIntyre, N.; Neef, N. and Lawson, G. H. K. (1993): Reproduction of porcine proliferative enteropathy with pure cultures of ileal symbiont intracellularis. I...
متن کاملDetection of Lawsonia intracellularis using immunomagnetic beads and ATP bioluminescence.
Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in various animals. The detection of L. intracellularis in clinical and environmental samples is necessary for the diagnosis of infection and epidemiological investigations. For the detection of L. intracellularis in fecal samples, we have developed an immunological method using immu...
متن کاملApplication of real-time PCR for detection of Lawsonia intracellularis and Brachyspira hyodysenteriae in fecal samples from pigs.
The aim of the study was to develop and validate real-time PCR method for the quantification of Lawsonia intracellularis and Brachyspira hyodysenteriae in porcine feces. Before the optimization process was performed two different extraction methods were compared to select the more efficient one. Based on the results achieved at this stage the boiling procedure was rejected and a commercially av...
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ژورنال
عنوان ژورنال: Journal of Veterinary Science
سال: 2000
ISSN: 1229-845X
DOI: 10.4142/jvs.2000.1.1.33